The Collaborative International Dictionary of English v.0.48:

Disaffect \Dis`af*fect"\, v. t. [imp. & p. p. Disaffected; p.
   pr. & vb. n. Disaffecting.]
   1. To alienate or diminish the affection of; to make
      unfriendly or less friendly; to fill with discontent and
      unfriendliness.
      [1913 Webster]

            They had attempted to disaffect and discontent his
            majesty's late army.                  --Clarendon.
      [1913 Webster]

   2. To disturb the functions of; to disorder.
      [1913 Webster]

            It disaffects the bowels.             --Hammond.
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   3. To lack affection for; to be alienated from, or indisposed
      toward; to dislike. [Obs.] --Bp. Hall.
      [1913 Webster]

WordNet (r) 3.0 (2006):

disaffect
    v 1: arouse hostility or indifference in where there had
         formerly been love, affection, or friendliness; "She
         alienated her friends when she became fanatically
         religious" [syn: estrange, alienate, alien,
         disaffect]